Difference between revisions of "Part:BBa K818300:Design"

 
(Design Notes)
 
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__NOTOC__
 
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<partinfo>BBa_K818300 short</partinfo>
 
<partinfo>BBa_K818300 short</partinfo>
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===Design Notes===
 
===Design Notes===
-
+
The suffix restriction site is PstI - SpeI instead of SpeI - PstI. This is important regarding cloning strategy.
 
+
 
+
  
 
===Source===
 
===Source===

Latest revision as of 13:16, 20 September 2012

Promoter alsT, repressed by TnrA during conversion of NH4 to Glutamine in B. subtilis.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 465
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 465
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 465
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 465
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 465
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The suffix restriction site is PstI - SpeI instead of SpeI - PstI. This is important regarding cloning strategy.

Source

This part is from Bacillus subtilis genome.

References