Difference between revisions of "Part:BBa K818300:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | - | + | The suffix restriction site is PstI - SpeI instead of SpeI - PstI. This is important regarding cloning strategy. |
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===Source=== | ===Source=== |
Latest revision as of 13:16, 20 September 2012
Promoter alsT, repressed by TnrA during conversion of NH4 to Glutamine in B. subtilis.
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 465
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 465
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 465
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 465
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 465
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The suffix restriction site is PstI - SpeI instead of SpeI - PstI. This is important regarding cloning strategy.
Source
This part is from Bacillus subtilis genome.