Difference between revisions of "Part:BBa K750001"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K750001 short</partinfo> | <partinfo>BBa_K750001 short</partinfo> | ||
− | This part contains Pbad promoter, rbs of 1.0 strength, LuxI producer and double terminator. Once the promoter Pbad is activated by arabinose, this part can produce LuxI protein. | + | == Description == |
+ | This part contains Pbad promoter, rbs of 1.0 strength, LuxI producer and double terminator. Once the promoter Pbad is activated by arabinose, this part can produce LuxI protein. The LuxI protein synthesize a small, diffusible acyl-homoserinelactone (AHL) signaling molecule. The AHL accumlates as the cell density increases, and it can bind with the LuxR protein. The LuxR-AHL can activate promoter LuxPR, which we used as the promoter of the GFP expression device(BBa_K750006) in our project. | ||
+ | |||
+ | == Perfomance == | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 14:14, 19 September 2012
LuxI expression device activated by arabinose(Regulated by RBS of 1.0 strength)
Description
This part contains Pbad promoter, rbs of 1.0 strength, LuxI producer and double terminator. Once the promoter Pbad is activated by arabinose, this part can produce LuxI protein. The LuxI protein synthesize a small, diffusible acyl-homoserinelactone (AHL) signaling molecule. The AHL accumlates as the cell density increases, and it can bind with the LuxR protein. The LuxR-AHL can activate promoter LuxPR, which we used as the promoter of the GFP expression device(BBa_K750006) in our project.
Perfomance
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 793
Illegal BamHI site found at 65 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]