Difference between revisions of "Part:BBa K759004"

Revision as of 13:38, 19 September 2012

phaA (beta-ketothiolase orgin from Ralstonia eutropha)

This gene codes the enzyme“Beta- ketothiolase (acetyl-CoA acetyltransferase)”which catalyzes the conversion of two acetyl-CoA molecules into acetoacetyl-CoA. It is an enzyme that catalyzes the first step of P3(HB) producing pathway like the picture below.

This gene was cloned from pGEM phbCAB which carries the synthetic operon genes (phaA,phaB,phaC) of Ralstonia eutropha. In the pGEM phbCAB phaA, we found a mutation in the 00th base according to the data base of NCBI.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 121
Illegal NgoMIV site found at 217
Illegal NgoMIV site found at 225
Illegal NgoMIV site found at 237
Illegal NgoMIV site found at 334
Illegal NgoMIV site found at 574
Illegal NgoMIV site found at 715
1000
COMPATIBLE WITH RFC[1000]

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 This gene codes the enzyme“Beta- ketothiolase (acetyl-CoA acetyltransferase)”which catalyzes the conversion of two acetyl-CoA molecules into acetoacetyl-CoA. It is an enzyme that catalyzes the first step of P3(HB) producing pathway like the picture below.
-This gene was cloned from pGEM phbCAB. The pGEM phbCAB carries the synthetic operon genes (phaA,phaB,phaC) of Ralstonia eutropha. In the pGEM phbCAB phaA, we found a mutation in the 00th base according to the data base of NCBI.
+This gene was cloned from pGEM phbCAB which carries the synthetic operon genes (phaA,phaB,phaC) of Ralstonia eutropha. In the pGEM phbCAB phaA, we found a mutation in the 00th base according to the data base of NCBI.
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