Difference between revisions of "Part:BBa K777121:Design"

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===Design Notes===
 
===Design Notes===
 
* ''yhjH'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers:
 
* ''yhjH'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers:
−
** Fwd: 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGATAAGGCAGGTTATCCAGC-3'
+
** Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgataaggcaggttatccagc-3'
−
** Rev: 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATAGCGCCAGAACCGCCGTATTCAGC-3'
+
** Rev: 5'-gtttcttcctgcagcggccgctactagtattatagcgccagaaccgccgtattcagc-3'
  
 
===Source===
 
===Source===

Revision as of 08:42, 19 September 2012

yhjH


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • yhjH was amplified from genomic DNA of E. coli DH10B via PCR using the following primers:
    • Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgataaggcaggttatccagc-3'
    • Rev: 5'-gtttcttcctgcagcggccgctactagtattatagcgccagaaccgccgtattcagc-3'

Source

  • The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1).

References

  • Paul K., Nieto V., Carlquist W.C., Blair D.F., Harshey R.M. The c-di-GMP Binding Protein YcgR Controls Flagellar Motor Direction and Speed to Affect Chemotaxis by a “Backstop Brake” Mechanism. Mol Cell. 2010. 38: 128–139.