Difference between revisions of "Part:BBa K784030"

Latest revision as of 21:48, 18 September 2012

pSP6+RBS+Alkaline Phosphatase (pHO)+Terminator

This part consists of SP6 RNA polymerase induced promoter followed by an RBS and the ‎Alkaline Phosphatase reporter gene (pHOA) from Citrobacter.‎ The part's purpose is to monitor the translation of the SP6 RNA polymerase with the Alkaline ‎Phosphatase gene which can be quantified with the PNP (para-nitrophenol phosphate) hydrolysis assay that has been ‎made by Arkin Lab group in 2006.‎ The Alkaline Phosphatase + RBS part is between XmaI and XhoI restriction sites and ‎therefore can be excised out by these enzymes.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 1506
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1506
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1506
Illegal XhoI site found at 18
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 1506
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1506
Illegal NgoMIV site found at 345
Illegal NgoMIV site found at 792
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K784030 short</partinfo>
-This part consists of T7 RNA polymerase induced promoter followed by an RBS and the &#8206;Alkaline Phosphatase reporter gene (pHOA) from Citrobacter.&#8206;
+This part consists of SP6 RNA polymerase induced promoter followed by an RBS and the &#8206;Alkaline Phosphatase reporter gene (pHOA) from Citrobacter.&#8206;
-The part's purpose is to monitor the translation of the T7 RNA polymerase with the Alkaline &#8206;Phosphatase gene which can be quantified with the [https://parts.igem.org/Arkin_JCA_PNPAssay  PNP (para-nitrophenol phosphate) hydrolysis assay] that has been &#8206;made by Arkin Lab group in 2006.&#8206;
+The part's purpose is to monitor the translation of the SP6 RNA polymerase with the Alkaline &#8206;Phosphatase gene which can be quantified with the [https://parts.igem.org/Arkin_JCA_PNPAssay  PNP (para-nitrophenol phosphate) hydrolysis assay] that has been &#8206;made by Arkin Lab group in 2006.&#8206;
 The Alkaline Phosphatase + RBS part is between XmaI and XhoI restriction sites and &#8206;therefore can be excised out by these enzymes.