Difference between revisions of "Part:BBa K861038"

 
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To find the optimal combination of those fatty acid degradation enzymes, we used IPTG induced promoter BBa_R0011 to express and extract those proteins, put them in different combination with substrates needed for fatty acid oxidation in a test tube to look for the combination that have best degradation capability. This part was used to produce protein FadA and FadB protein in <i>Salmonella enterica LT2</i>.
  
 
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Revision as of 17:37, 18 September 2012

IPTG induced S-FadA and S-FadB

To find the optimal combination of those fatty acid degradation enzymes, we used IPTG induced promoter BBa_R0011 to express and extract those proteins, put them in different combination with substrates needed for fatty acid oxidation in a test tube to look for the combination that have best degradation capability. This part was used to produce protein FadA and FadB protein in Salmonella enterica LT2.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 168
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2391
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1621