Difference between revisions of "Part:BBa K731600:Design"

 
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<partinfo>BBa_K731600 short</partinfo>
 
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===Design Notes===
 
===Design Notes===
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The nucleotide sequence of CysDes was optimized by Genscript, thus it does not correspond to the natural sequence found in Treponema denticola.  
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The nucleotidic sequence of CysDes was optimized by Genscript, thus it does not correspond to the natural sequence found in ''Treponema denticola''.  
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The sequence of the lac operator corresponds to the sequence found in pET21b.
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The sequence of the lac operator corresponds to the pET21b sequence.
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===Source===
 
===Source===

Revision as of 12:46, 17 September 2012

Cysteine Desulfhydrase (CysDes)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 399
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The nucleotidic sequence of CysDes was optimized by Genscript, thus it does not correspond to the natural sequence found in Treponema denticola. The sequence of the lac operator corresponds to the pET21b sequence.

Source

CysDes nucleotide sequence was synthesized by Genscript and subcloned into psB1C3 to create part BBa_K731600.

References