Difference between revisions of "Part:BBa K784000:Experience"
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We have used the part inside a BL21 strain of ''E.coli'' which has an endogenouse PET system. <br> | We have used the part inside a BL21 strain of ''E.coli'' which has an endogenouse PET system. <br> | ||
In the presence of IPTG inducer, T7 RNAP is expressed and activates the part promoter which expresses the Alkaline Phosphatase gene (phoA).<br> | In the presence of IPTG inducer, T7 RNAP is expressed and activates the part promoter which expresses the Alkaline Phosphatase gene (phoA).<br> | ||
| − | We checked the absorbance resulted from the PNP assay in raising concetrations of the inducer (IPTG) and the results are before you in the graph: <br> | + | We checked the absorbance resulted from the [https://parts.igem.org/Arkin_JCA_PNPAssay PNP (para-nitrophenol phosphate) hydrolysis assay] in raising concetrations of the inducer (IPTG) and the results are before you in the graph: <br> |
As you can see in the graph, raising concentrations of the inducer results in raising absorbance, which means raising concentration of the Alkaline Phosphatase enzyme. | As you can see in the graph, raising concentrations of the inducer results in raising absorbance, which means raising concentration of the Alkaline Phosphatase enzyme. | ||
Revision as of 10:43, 17 September 2012
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Applications of BBa_K784000
We have used the part inside a BL21 strain of E.coli which has an endogenouse PET system.
In the presence of IPTG inducer, T7 RNAP is expressed and activates the part promoter which expresses the Alkaline Phosphatase gene (phoA).
We checked the absorbance resulted from the PNP (para-nitrophenol phosphate) hydrolysis assay in raising concetrations of the inducer (IPTG) and the results are before you in the graph:
As you can see in the graph, raising concentrations of the inducer results in raising absorbance, which means raising concentration of the Alkaline Phosphatase enzyme.
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UNIQ3f992712003a78ae-partinfo-00000000-QINU UNIQ3f992712003a78ae-partinfo-00000001-QINU