Difference between revisions of "Part:BBa K731500"
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<partinfo>BBa_K731500 short</partinfo> | <partinfo>BBa_K731500 short</partinfo> | ||
| − | This composite part consist of | + | This composite part consist of [https://parts.igem.org/Part:BBa_K731300 BBa_K731300] part (lacI-lacIq) and a ''tac'' promoter followed |
by a ''lac'' operator in the forward direction. It is a ready to use composite part and it allows the expression of a gene of interest with a strong ''E.coli'' promoter and under the control of IPTG. The ''lac'' operator decreases basal levels of expression. | by a ''lac'' operator in the forward direction. It is a ready to use composite part and it allows the expression of a gene of interest with a strong ''E.coli'' promoter and under the control of IPTG. The ''lac'' operator decreases basal levels of expression. | ||
Revision as of 10:30, 17 September 2012
[LacI + LacIq promoter] reverse + [tac promoter + lac operator] forward
This composite part consist of BBa_K731300 part (lacI-lacIq) and a tac promoter followed by a lac operator in the forward direction. It is a ready to use composite part and it allows the expression of a gene of interest with a strong E.coli promoter and under the control of IPTG. The lac operator decreases basal levels of expression.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]