Difference between revisions of "Part:BBa K774001:Experience"

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<partinfo>BBa_K774001 1</partinfo>
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<I>RussellGritton</I>
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This part was ligated with [https://parts.igem.org/Part:BBa_E0420 Part:BBa_E0420]
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Enter the review inofrmation here.
 
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[[Image:NRPMBPhoto.jpg | right | 200px ]]
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This part was ligated with [https://parts.igem.org/wiki/index.php/Part:BBa_E0420 BBa_E0420] to give a ribosome binding site (RBS) and cyan fluorescent protein (CFP) reporter. ''Escherichia coli'' was then transformed by the part ligated with BBa_E0420 and grown in media also containing potassium nitrate (as a source of nitrates in order to induce promoter activity) at concentrations of 0 mM, 10 mM, 50 mM and 100 mM. The ''E. coli'' was grown for 6 hours and then added to eppendorf tubes and spun down in a centrifuge in order to produce a pellet. The four samples were then viewed under a UV box to assess for fluorescence; as the accompanying photograph shows the sample at 0 mM potassium nitrate did not fluoresce, however those at 10, 50 and 100 mM potassium nitrate did fluoresce. They also appeared to fluoresce at the same strength, suggesting that 10 mM was equal to or above the maximum sensitivity level of this part.
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Revision as of 19:03, 16 September 2012

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K774001

User Reviews

UNIQabebd56de7a33fb7-partinfo-00000000-QINU UNIQabebd56de7a33fb7-partinfo-00000001-QINU

No review score entered. RussellGritton

NRPMBPhoto.jpg

This part was ligated with BBa_E0420 to give a ribosome binding site (RBS) and cyan fluorescent protein (CFP) reporter. Escherichia coli was then transformed by the part ligated with BBa_E0420 and grown in media also containing potassium nitrate (as a source of nitrates in order to induce promoter activity) at concentrations of 0 mM, 10 mM, 50 mM and 100 mM. The E. coli was grown for 6 hours and then added to eppendorf tubes and spun down in a centrifuge in order to produce a pellet. The four samples were then viewed under a UV box to assess for fluorescence; as the accompanying photograph shows the sample at 0 mM potassium nitrate did not fluoresce, however those at 10, 50 and 100 mM potassium nitrate did fluoresce. They also appeared to fluoresce at the same strength, suggesting that 10 mM was equal to or above the maximum sensitivity level of this part.