Difference between revisions of "Part:BBa K861161"
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− | Coding region for cAMP receptor protein(CRP; also known as catabolite activator protein, CAP). cAMP receptor protein is a DNA-binding transcriptional dual regulator in bacteria. CRP protein binds cAMP | + | Coding region for cAMP receptor protein(CRP; also known as catabolite activator protein, CAP). cAMP receptor protein is a DNA-binding transcriptional dual regulator in bacteria. CRP protein binds cAMP, which causes a conformational change that allows CRP to bind tightly to a specific DNA site in the promoters of the genes it controls. Often, CRP is considered as an activator. In our design, we successfully turn the activator into repressor by altering the position of binding site in the CRP regulated promoter. This part contains CRP with a RBS and double terminators. |
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Revision as of 06:09, 16 September 2012
CRP with a RBS and a terminator
Coding region for cAMP receptor protein(CRP; also known as catabolite activator protein, CAP). cAMP receptor protein is a DNA-binding transcriptional dual regulator in bacteria. CRP protein binds cAMP, which causes a conformational change that allows CRP to bind tightly to a specific DNA site in the promoters of the genes it controls. Often, CRP is considered as an activator. In our design, we successfully turn the activator into repressor by altering the position of binding site in the CRP regulated promoter. This part contains CRP with a RBS and double terminators.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]