Difference between revisions of "Part:BBa K784005"
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This part has been originally designed by Sean A. Lynch and Justin P. Gallivan. The part is a riboswitch which is comprised of an aptamer which recognizes the ligand, theophylline. The riboswitch also contains an RBS. In the absence of theophylline, there is extensive base pairing in the 5' untranslated region (UTR) which blocks the RBS. This base pairing prevents translation of a gene downstream to the riboswitch. In the presence of theophylline, the transcript adopts a secondary structure in which the RBS is exposed, allowing translation to occur.<br> | This part has been originally designed by Sean A. Lynch and Justin P. Gallivan. The part is a riboswitch which is comprised of an aptamer which recognizes the ligand, theophylline. The riboswitch also contains an RBS. In the absence of theophylline, there is extensive base pairing in the 5' untranslated region (UTR) which blocks the RBS. This base pairing prevents translation of a gene downstream to the riboswitch. In the presence of theophylline, the transcript adopts a secondary structure in which the RBS is exposed, allowing translation to occur.<br> | ||
For this part to work properly, it must be directly fused to the starting codon of the downstream coding region.<br> | For this part to work properly, it must be directly fused to the starting codon of the downstream coding region.<br> | ||
| − | This part is a duplicate of [[Part:BBa_J89000|BBa_J89000]] but with additional sequence notations and additional sequence upstream to the theophylline aptamer. | + | This part is a duplicate of [[Part:BBa_J89000|BBa_J89000]] but with additional sequence notations and additional sequence upstream to the theophylline aptamer.<br> |
| + | The physical DNA of this part hasn't been sent to the registry. However, a fusion product of this part with mCherry is available as [[Part:BBa_K784006|BBa_K784006]]. | ||
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Revision as of 18:08, 15 September 2012
Theophylline riboswitch 12.1
This part has been originally designed by Sean A. Lynch and Justin P. Gallivan. The part is a riboswitch which is comprised of an aptamer which recognizes the ligand, theophylline. The riboswitch also contains an RBS. In the absence of theophylline, there is extensive base pairing in the 5' untranslated region (UTR) which blocks the RBS. This base pairing prevents translation of a gene downstream to the riboswitch. In the presence of theophylline, the transcript adopts a secondary structure in which the RBS is exposed, allowing translation to occur.
For this part to work properly, it must be directly fused to the starting codon of the downstream coding region.
This part is a duplicate of BBa_J89000 but with additional sequence notations and additional sequence upstream to the theophylline aptamer.
The physical DNA of this part hasn't been sent to the registry. However, a fusion product of this part with mCherry is available as BBa_K784006.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 15
- 1000COMPATIBLE WITH RFC[1000]