Difference between revisions of "Part:BBa K823021"
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This part is a reporter vector for Bacillus subtilis. It integrates at the amyE locus. It has a ampicillin resistance for cloning in E.coli and chloramphenicol resistance for B.subtilis. The multiple cloning site is followed by a lacZ gene for reporter assays. This backbone is a ''BioBricked'' version of the ''B. subtilis'' vector pAC6.
 
This part is a reporter vector for Bacillus subtilis. It integrates at the amyE locus. It has a ampicillin resistance for cloning in E.coli and chloramphenicol resistance for B.subtilis. The multiple cloning site is followed by a lacZ gene for reporter assays. This backbone is a ''BioBricked'' version of the ''B. subtilis'' vector pAC6.
  
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[[image:https://static.igem.org/mediawiki/2012/0/04/LMU-Munich-PSBBs1C-lacZ.png | heigth=50]]
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[image:https://static.igem.org/mediawiki/2012/0/04/LMU-Munich-PSBBs1C-lacZ.png | heigth=50]
  
 
This BioBrick is part of the LMU 2012 igem project [http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks '''''Bacillus''B'''io'''B'''rick'''B'''ox]
 
This BioBrick is part of the LMU 2012 igem project [http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks '''''Bacillus''B'''io'''B'''rick'''B'''ox]

Revision as of 13:22, 15 September 2012

pSBBs1C-lacZ (lacZ reporter vector for B. subtilis)

This part is a reporter vector for Bacillus subtilis. It integrates at the amyE locus. It has a ampicillin resistance for cloning in E.coli and chloramphenicol resistance for B.subtilis. The multiple cloning site is followed by a lacZ gene for reporter assays. This backbone is a BioBricked version of the B. subtilis vector pAC6.

[image:LMU-Munich-PSBBs1C-lacZ.png | heigth=50]

This BioBrick is part of the LMU 2012 igem project [http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks BacillusBioBrickBox]

Reference: [http://www.ncbi.nlm.nih.gov/pubmed/11902727 Stülke et al.]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 9771
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 9777
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 9771
    Illegal BglII site found at 8313
    Illegal BamHI site found at 22
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 9771
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 9771
    Plasmid lacks a suffix.
    Illegal XbaI site found at 9786
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 4562
    Illegal NgoMIV site found at 6888
    Illegal NgoMIV site found at 8688
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 4738
    Illegal SapI.rc site found at 5820