Difference between revisions of "Part:BBa K731400:Design"

(→‎Design Notes)
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The part was designed so that LacI transcription/translation does not affect the transcription/translation of CysDes. In other words LacI+lacIq cassette (BBa_K731300) is in the reverse direction. Tac promoter + Lac operator + CysDes are in the forward direction.
 
The part was designed so that LacI transcription/translation does not affect the transcription/translation of CysDes. In other words LacI+lacIq cassette (BBa_K731300) is in the reverse direction. Tac promoter + Lac operator + CysDes are in the forward direction.
  
−
The nucleotide sequence of CysDes [https://parts.igem.org/Part:BBa_K731030 Part BBa_K731300] used to create this composite part has been codon optimised before Gene Synthesis by Genescript.
+
The nucleotide sequence of CysDes [https://parts.igem.org/Part:BBa_K731300 Part BBa_K731300] used to create this composite part has been codon optimised before Gene Synthesis by Genescript.
  
 
===Source===
 
===Source===

Revision as of 16:15, 14 September 2012

IPTG inducible Cysteine desulfhydrase (CysDes)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1560
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part was designed so that LacI transcription/translation does not affect the transcription/translation of CysDes. In other words LacI+lacIq cassette (BBa_K731300) is in the reverse direction. Tac promoter + Lac operator + CysDes are in the forward direction.

The nucleotide sequence of CysDes Part BBa_K731300 used to create this composite part has been codon optimised before Gene Synthesis by Genescript.

Source

K731300 and K731600

References