Difference between revisions of "Part:BBa K801999:Design"

Revision as of 10:15, 13 September 2012

Test page for standardized BioBrick part descriptions

Assembly Compatibility:

Keywords:

Abbreviations:

Other versions of this BioBrick:

Cloning details:

Protein coding:

Enzymatic activity:

Cytotoxicity:

Source:

Organism:

Literature references:

Sequence references:

Structure reference:

Test page for standardized BioBrick part descriptions

Keywords: fluorescent, reporter, chromophore, luminescence, bioluminescence, photoprotein

Used abbreviations:

Other versions of this BioBrick:

The BioBrick BBa_I757008 encodes a yellow fluorescent protein (mVenus) derived from GFP
The BioBrick BBa_I757008 encodes GFP in RFC25 for protein fusions

Cloning details:

Protein coding:

Green Fluorescent Protein [Nucleotide 1 to 714]
The protein has the amino acid replacements Ser65Thr in order to increase fluorescence, photostability and to shift the major excitation peak to 488 nm. (see Heim et al., 1995)
The protein encoded is posttranslationally modified by a cross-link between Ser65 and Gly67 to form the chromophore.

Enzymatic activity: none

Cytotoxicity: none

Source:

Amplified from plasmid: pSB1C3-GFP-generator, provided by Osamu Shimomura, Boston University School of Medicine, USA

Forward Primer:
5'- ATGATGATGATG - 3'
Reverse Primer:
5'- ATGATGATGATG - 3'

Organism:

Literature references:

[http://www.ncbi.nlm.nih.gov/pubmed/20010584 Pubmed: Prasher, 1995: Using GFP to see the light.(Review)]
[http://www.ncbi.nlm.nih.gov/pubmed/7854443 Pubmed: Heim, 1995: Improved green fluorescence.(Reference for Chromophor)]

Seqeuence references:

[http://www.ncbi.nlm.nih.gov/nuccore/X83959.1 GeneBank: A.victoria mRNA for green fluorescent protein (ID:gfp1)]
[http://www.ebi.ac.uk/interpro/IEntry?ac=IPR011584 Interpro: Green fluorescent protein-related ]
[http://www.uniprot.org/uniprot/P42212 Uniprot: Green fluorescent protein]
[http://pfam.sanger.ac.uk/family/PF01353 Pfam: Green fluorescent protein]

Structure reference:

[http://www.rcsb.org/pdb/explore/explore.do?structureId=1EMA PDB: Green Fluorescent Protein from Aequorea victoria]

@@ Line 81: / Line 81: @@
 '''Other versions of this BioBrick:'''
-*The BioBrick BBa_I757008 encodes a yellow fluorescent protein (mVenus) derived from GFP.
+*The BioBrick BBa_I757008 encodes a yellow fluorescent protein (mVenus) derived from GFP
-*The BioBrick BBa_I757008 encodes GFP in RFC25 for protein fusions.
+*The BioBrick BBa_I757008 encodes GFP in RFC25 for protein fusions
 '''Cloning details:'''<br>
-*Part was designed in RFC10.
+*Part was designed in RFC10
 *Mutation G381A to delete XbaI restriction site
-*The correctness of the part was checked by sequencing.
+*The correctness of the part was checked by sequencing
 '''Protein coding:'''<br>
-*Green Fluorescent Prtein [Nucleotide 1 to 714]
+*Green Fluorescent Protein [Nucleotide 1 to 714]
-*The protein has the amino acid replacements Ser65Thr in order to increased fluorescence, photostability, and to shift the major excitation peak to 488 nm. (see Heim et all., 1995)
+*The protein has the amino acid replacements Ser65Thr in order to increase fluorescence, photostability and to shift the major excitation peak to 488 nm. (see Heim et al., 1995)
-*The protein encoded is posttranslationally modified by a cross-link between Ser65 and Gly67 to form the chromophor.
+*The protein encoded is posttranslationally modified by a cross-link between Ser65 and Gly67 to form the chromophore.
 '''Enzymatic activity:'''