Difference between revisions of "Part:BBa K801999:Design"
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===Source=== | ===Source=== | ||
'''Source:'''<br> | '''Source:'''<br> | ||
− | <!--Amplified from commercial system: plasmid name, system name, company name<br>--> | + | <!--*Amplified from commercial system: plasmid name, system name, company name<br>--> |
− | <!--Amplified from plasmid: p???, provided by | + | <!--*Amplified from plasmid: p???, provided by ?name_of_person?, ?institute/university?, ?country?--> |
− | <!--Preexisting BioBrick Bba_number | + | <!--*Preexisting BioBrick ?Bba_number?--> |
− | <!--cDNA Clone: | + | <!--*cDNA Clone: ?clone_name?, ?company_name?--> |
− | <!-- | + | <!--*Synthesized by ?company_name?.--> |
+ | |||
+ | <!--'''Forward Primer:'''<br><code>5'- ??? - 3'</code><br>--> | ||
+ | |||
+ | <!--'''Reverse Primer:'''<br><code>5'- ??? - 3'</code><br>--> | ||
'''Organism:'''<br> | '''Organism:'''<br> | ||
− | <!--Genesequence derived from '' | + | <!--*Genesequence derived from ''?organism_name?''--> |
− | <!--Codonoptimized for '' | + | <!--*Codonoptimized for ''?organism_name?''--> |
===References=== | ===References=== |
Revision as of 20:52, 11 September 2012
Test page for standardized BioBrick part descriptions
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Keywords:
Abbreviations:
Design Notes
Other versions of this BioBrick:
Cloning details:
Protein coding:
Enzymatic activity:
Cytotoxicity:
Source
Source:
Organism:
References
Literature references:
Seqeuence references:
Structure reference:
Test page for standardized BioBrick part descriptions
Keywords:
fluorescent, reporter, chromophore, luminescence, bioluminescence, photoprotein
Used abbreviations:
- GFP = Green Fluorescent Protein
Design Notes
Other versions of this BioBrick:
- The BioBrick BBa_I757008 encodes a yellow fluorescent protein (mVenus) derived from GFP.
- The BioBrick BBa_I757008 encodes GFP in RFC25 for protein fusions.
Cloning details:
- Part was designed in RFC10.
- Mutation G381A to delete XbaI restriction site
- The correctness of the part was checked by sequencing.
Protein coding:
- Green Fluorescent Prtein [Nucleotide 1 to 714]
- The protein has the amino acid replacements Ser65Thr in order to increased fluorescence, photostability, and to shift the major excitation peak to 488 nm. (see Heim et all., 1995)
- The protein encoded is posttranslationally modified by a cross-link between Ser65 and Gly67 to form the chromophor.
Enzymatic activity: none
Cytotoxicity: none
Source
Source:
- Amplified from plasmid: pSB1C3-GFP-generator, provided by A. Skerra, TU Munich, Germany
Forward Primer:
5'- ??? - 3'
Reverse Primer:
5'- ??? - 3'
Organism:
- Genesequence derived from Aequorea victoria
- Codonoptimized for Escherichia coli
References
Literature references:
- [http://www.ncbi.nlm.nih.gov/pubmed/20010584 Pubmed: Prasher, 1995: Using GFP to see the light.(Review)]
- [http://www.ncbi.nlm.nih.gov/pubmed/7854443: Heim, 1995: Improved green fluorescence.(Reference for Chromophor)]
Seqeuence references:
- [http://www.ncbi.nlm.nih.gov/nuccore/X83959.1 GeneBank: A.victoria mRNA for green fluorescent protein (ID:gfp1)]
- [http://www.ebi.ac.uk/interpro/IEntry?ac=IPR011584 Interpro: Green fluorescent protein-related ]
- [http://www.uniprot.org/uniprot/P42212 Uniprot: Green fluorescent protein]
- [http://pfam.sanger.ac.uk/family/PF01353 Pfam: Green fluorescent protein]
Structure reference:
- [http://www.rcsb.org/pdb/explore/explore.do?structureId=1EMA PDB: Green Fluorescent Protein from Aequorea victoria]