Difference between revisions of "Part:BBa K801999:Design"

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'''Cloning details:'''<br>
 
'''Cloning details:'''<br>
 
*Part was designed in RFC10.
 
*Part was designed in RFC10.
−
*Mutation C889G to delete XbaI restriction site"<br>-->
+
*Mutation G381A to delete XbaI restriction site
−
<!--"Part was truncated upstream/downstream compared to template in order to ???"<br>-->
+
*The correctness of part was checked by sequencing.
−
<!--"The correctness of part was checked by sequencing" or "... was not yet checked by sequencing"<br>-->
+
  
 
'''Protein coding:'''<br>
 
'''Protein coding:'''<br>
−
<!--"The BioBrick does not encode a protein" or "Name of gene product [Nucleotide 1 to ???]"<br>-->
+
*Green Fluorescent Prtein [Nucleotide 1 to 714]>
−
<!--"The protein has the amino acid replacements Cys59Tyr in order to..."<br>-->
+
*The protein has the amino acid replacements Ser65Thr in order to increased fluorescence, photostability, and to shift the major excitation peak to 488 nm. (see Heim et all., 1995)
−
<!--"The protein encoded is posttranslationally modified..."<br>-->
+
*The protein encoded is posttranslationally modified by a cross-link between Ser65 and Gly67 to form the chromophor.
  
−
'''Enzymatic activity:'''<br>
+
'''Enzymatic activity:'''
−
<!--none/EC-number ?.?.?.?<br>-->
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*none
  
−
'''Cytotoxicity:'''<br>
+
'''Cytotoxicity:'''
−
<!--none/not known/cytotoxic for ''organim name''<br>-->
+
*none
  
 
===Source===
 
===Source===
 
'''Source:'''
 
'''Source:'''
−
Synthesized<br>
+
*Synthesized<br>
  
−
'''Organism:'''<br>
+
'''Organism:'''
−
<!--Genesequence derived from ''Aequorea victoria''<br>-->
+
*Genesequence derived from ''Aequorea victoria''
−
<!--Codonoptimized for ''Escherichia coli''<br>-->
+
*Codonoptimized for ''Escherichia coli''
  
 
===References===
 
===References===

Revision as of 19:48, 11 September 2012

Test page for standardized BioBrick part descriptions


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Keywords:
Abbreviations:

Design Notes

Other versions of this BioBrick:

Cloning details:

Protein coding:

Enzymatic activity:

Cytotoxicity:

Source

Source:

Organism:

References

Literature references:

Seqeuence references:

Structure reference:


TUM12-Test page1.png


Test page for standardized BioBrick part descriptions


Keywords: fluorescent, reporter
Abbreviations:

  • GFP = Green Fluorescent Protein


Design Notes

Other versions of this BioBrick:

  • The BioBrick Bba_??? encodes a yellow fluorescent protein (mVenus)derived from GFP.
  • The BioBrick Bba_??? encodes GFP in RFC25 for protein fusions.

Cloning details:

  • Part was designed in RFC10.
  • Mutation G381A to delete XbaI restriction site
  • The correctness of part was checked by sequencing.

Protein coding:

  • Green Fluorescent Prtein [Nucleotide 1 to 714]>
  • The protein has the amino acid replacements Ser65Thr in order to increased fluorescence, photostability, and to shift the major excitation peak to 488 nm. (see Heim et all., 1995)
  • The protein encoded is posttranslationally modified by a cross-link between Ser65 and Gly67 to form the chromophor.

Enzymatic activity:

  • none

Cytotoxicity:

  • none

Source

Source:

  • Synthesized

Organism:

  • Genesequence derived from Aequorea victoria
  • Codonoptimized for Escherichia coli

References

Literature references:

  • [http://www.ncbi.nlm.nih.gov/pubmed/20010584 Pubmed: Prasher, 1995: Using GFP to see the light.(Review)]
  • [http://www.ncbi.nlm.nih.gov/pubmed/20010584 Pubmed: Prasher, 1995: Using GFP to see the light.(Review)]

Seqeuence references:

  • [http://www.ncbi.nlm.nih.gov/nuccore/X83959.1 GeneBank: A.victoria mRNA for green fluorescent protein (ID:gfp1)]
  • [http://www.ebi.ac.uk/interpro/IEntry?ac=IPR011584 Interpro: Green fluorescent protein-related ]
  • [http://www.uniprot.org/uniprot/P42212 Uniprot: Green fluorescent protein]
  • [http://pfam.sanger.ac.uk/family/PF01353 Pfam: Green fluorescent protein]

Structure reference:

  • [http://www.rcsb.org/pdb/explore/explore.do?structureId=1EMA PDB: Green Fluorescent Protein from Aequorea victoria]