Difference between revisions of "Part:BBa K777000:Design"

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Fwd: Tar for + prefix: 5´ actgAATTCgcggccgcttCTAGAtgattaaccgtatccgcgtagtc 3´
 
Fwd: Tar for + prefix: 5´ actgAATTCgcggccgcttCTAGAtgattaaccgtatccgcgtagtc 3´
  
−
Rv: Tar rev + suffix: 5´ tcCTGCAGcggccgctACTAGTcaaaatgtttcccagtttggatc 3´
+
Rev: Tar rev + suffix: 5´ tcCTGCAGcggccgctACTAGTcaaaatgtttcccagtttggatc 3´
  
 
===Source===
 
===Source===

Revision as of 19:34, 11 September 2012

Tar receptor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1282
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 111


Design Notes

One XbaI site was found at position 420. The restriction site was changed from TCTAGt to CCTAGt via QuikChange PCR.

Primers: Base pairs in caps represent the respective restriction sites followed by either prefix or suffix. Primers were provided by SIGMA.

Fwd: Tar for + prefix: 5´ actgAATTCgcggccgcttCTAGAtgattaaccgtatccgcgtagtc 3´

Rev: Tar rev + suffix: 5´ tcCTGCAGcggccgctACTAGTcaaaatgtttcccagtttggatc 3´

Source

The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B chromosome (NC_010473.1).

References