Difference between revisions of "Part:BBa K777000:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K777000 short</partinfo>
 
<partinfo>BBa_K777000 short</partinfo>
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===Design Notes===
 
===Design Notes===
 
One XbaI site was found at position 420. The restriction site was changed from TCTAGt to CCTAGt via QuikChange PCR.
 
One XbaI site was found at position 420. The restriction site was changed from TCTAGt to CCTAGt via QuikChange PCR.
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Primers: Note: Base pairs in caps represent the respective restriction sites followed by either prefix or suffix. Primers were provided by SIGMA.
  
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Fwd: Tar for + prefix: 5´ actgAATTCgcggccgcttCTAGAtgattaaccgtatccgcgtagtc 3´
  
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Rv: Tar rev + suffix: 5´ tcCTGCAGcggccgctACTAGTcaaaatgtttcccagtttggatc 3´
  
 
===Source===
 
===Source===

Revision as of 19:30, 11 September 2012

Tar receptor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1282
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 111


Design Notes

One XbaI site was found at position 420. The restriction site was changed from TCTAGt to CCTAGt via QuikChange PCR. Primers: Note: Base pairs in caps represent the respective restriction sites followed by either prefix or suffix. Primers were provided by SIGMA.

Fwd: Tar for + prefix: 5´ actgAATTCgcggccgcttCTAGAtgattaaccgtatccgcgtagtc 3´

Rv: Tar rev + suffix: 5´ tcCTGCAGcggccgctACTAGTcaaaatgtttcccagtttggatc 3´

Source

The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B chromosome (NC_010473.1).

References