Difference between revisions of "Part:BBa K729002"

(Characterisation)
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===Characterisation===
 
===Characterisation===
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Plastic degradation is mediated via a laccase protein. As such, we will be using a laccase enzymatic activity assay to determine the production of laccase. Laccase catalyses the oxidation of <span class="footnote" title="Syringaldazine">syringaldazine</span>, a reaction that exhibits an observable OD change at 530nm. A sample of syringaldazine can be used as a blank in a spectrophotometer, against a sample containing syringaldazine and our laccase sample, allowing the rate of oxidation to be measured, and hence the enzymatic activity of laccase.
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In order to determine the effectiveness of laccase in degrading plastic, we will expose strips of various types of plastic to the laccase expressing bacteria, before viewing the strips under a scanning electron microscope. This will allow us to compare the pitting in plastic samples treated with laccase, to the untreated samples, allowing us to determine the extent of plastic degradation.
  
 
===Results===
 
===Results===

Revision as of 14:40, 11 September 2012

Laccase for Polyethylene Degradation

Laccase for the degradation of polyethylene and organic pollutants. Sequence pending.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 225
  • 1000
    COMPATIBLE WITH RFC[1000]



Description

Characterisation

Plastic degradation is mediated via a laccase protein. As such, we will be using a laccase enzymatic activity assay to determine the production of laccase. Laccase catalyses the oxidation of syringaldazine, a reaction that exhibits an observable OD change at 530nm. A sample of syringaldazine can be used as a blank in a spectrophotometer, against a sample containing syringaldazine and our laccase sample, allowing the rate of oxidation to be measured, and hence the enzymatic activity of laccase.

In order to determine the effectiveness of laccase in degrading plastic, we will expose strips of various types of plastic to the laccase expressing bacteria, before viewing the strips under a scanning electron microscope. This will allow us to compare the pitting in plastic samples treated with laccase, to the untreated samples, allowing us to determine the extent of plastic degradation.

Results

Modelling

Conclusion