Difference between revisions of "Part:BBa K823022"

Revision as of 14:12, 11 September 2012

pSB_Bs4S: Empty backbone for integration into Bacillus subtilis thrC locus

This part is an empty backbone vector for the usage in Bacillus subtilis. It integrates in the thrC locus and can be selected with Spectinomycin cassette. It has a ampicillin resistance for cloning in E.coli. It originates from the Bacillus vector pDG1731.

This BioBrick is part of the LMU 2012 Bacillus BioBrick Box project: http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks

Reference: [http://www.ncbi.nlm.nih.gov/pubmed/8973347 Guérout-Fleury et al.]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 4552
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 4558
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 4552
Illegal BamHI site found at 4534
Illegal XhoI site found at 1011
Illegal XhoI site found at 1929
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found at 4552
Illegal suffix found at 2
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found at 4552
Plasmid lacks a suffix.
Illegal XbaI site found at 4567
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NgoMIV site found at 1893
Illegal NgoMIV site found at 3032
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 2856
Illegal SapI.rc site found at 605

@@ Line 6: / Line 6: @@
 This BioBrick is part of the LMU 2012 Bacillus BioBrick Box project:
 http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks
+Reference: [http://www.ncbi.nlm.nih.gov/pubmed/8973347 Guérout-Fleury ''et al.'']
 <!-- Add more about the biology of this part here