Difference between revisions of "Part:BBa K823019"
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suffix: ACCGGTTAATACTAGTAGCGGCCGCTGCAGT
 
suffix: ACCGGTTAATACTAGTAGCGGCCGCTGCAGT
  
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We used this ''lacZ'' gene to evaluate our promoters.
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[[Image:LacZ plate.png|thumb|center|400px|lacZ under the control of Pspac in pSB1C3. Plate was induced with xylose.]]
  
  

Revision as of 09:09, 10 September 2012

LacZ (RBS+Freiburg standard)

This lacZ gene is derived from the Bacillus reporter vector pAC6. It is constructed in the Freiburg standard with RBS.


prefix: GAATTCCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGC

suffix: ACCGGTTAATACTAGTAGCGGCCGCTGCAGT



We used this lacZ gene to evaluate our promoters.

lacZ under the control of Pspac in pSB1C3. Plate was induced with xylose.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]