Difference between revisions of "Part:BBa K823028:Design"
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===Design Notes=== | ===Design Notes=== | ||
codon optimized for Bacillus subtilis | codon optimized for Bacillus subtilis | ||
| + | |||
| + | prefix: (next nucleotide is then the a from atg); Includes RBS with correct spacing | ||
| + | |||
| + | GAGCAGAATTCGCGGCCGCTTCTAGAGTAAGGAGGAACTACT | ||
| + | |||
| + | suffix: (directly after the TAA) | ||
| + | TACTAGTAGCGGCCGCTGCAGGCTGA | ||
===Source=== | ===Source=== | ||
Revision as of 11:56, 30 August 2012
luc luciferase + RBS, codon optimized for Bacillus subtilis
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 625
Illegal SapI site found at 853
Design Notes
codon optimized for Bacillus subtilis
prefix: (next nucleotide is then the a from atg); Includes RBS with correct spacing
GAGCAGAATTCGCGGCCGCTTCTAGAGTAAGGAGGAACTACT
suffix: (directly after the TAA)
TACTAGTAGCGGCCGCTGCAGGCTGA
Source
synthesis by gene art