Difference between revisions of "Part:BBa K731500:Design"
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<partinfo>BBa_K731500 short</partinfo> | <partinfo>BBa_K731500 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | + | This part is composed of K731300 + a tac promoter followed by a | |
| − | + | Lac operator that corresponds to the lac operator sequence found in pET21b. | |
| − | + | tac promoter and lac operator were amplified by PCR starting from a synthesized gene composed of prefix-lacI-lacIq-Ptac-lacO-CysDes-suffix in pUC57. | |
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===Source=== | ===Source=== | ||
Revision as of 08:10, 29 August 2012
[LacI + LacIq promoter] reverse + [tac promoter + lac operator] forward
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is composed of K731300 + a tac promoter followed by a Lac operator that corresponds to the lac operator sequence found in pET21b. tac promoter and lac operator were amplified by PCR starting from a synthesized gene composed of prefix-lacI-lacIq-Ptac-lacO-CysDes-suffix in pUC57.
Source
pET21b