Difference between revisions of "Part:BBa K524204:Design"
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===References=== | ===References=== | ||
| + | Datsenko KA, Wanner BL.(2000).One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products, Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6640-5 | ||
Latest revision as of 16:48, 26 August 2012
pTE
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Lox or FRT sites were not included in this plasmid, partly because no qualified BioBricks were available (functionality of parts for lox66 and lox71 were questioned, and FRT sites were not available from DNA Kit Plates).
Source
The pTE plasmid was formerly the BBa_S05033 insert that resided in pSB1A2, which was excised out with XbaI and SpeI, and then self-ligated to generate the plasmid.
References
Datsenko KA, Wanner BL.(2000).One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products, Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6640-5