Difference between revisions of "Part:BBa K524203:Design"
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===References=== | ===References=== | ||
| + | Tucker WT, Miller CA, Cohen SN. Structural and functional analysis of the par region of the pSC101 plasmid. Cell. 1984;38:191–201. | ||
Revision as of 15:09, 26 August 2012
pSC101 heat sensitive origin of replication with deletion in par locus
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 1709
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The par locus was deleted through excision of BBa_K524000 by SspI and StuI, purification of desired fragments and then
Source
Modified from BBa_K524000
References
Tucker WT, Miller CA, Cohen SN. Structural and functional analysis of the par region of the pSC101 plasmid. Cell. 1984;38:191–201.