Difference between revisions of "Part:BBa K731722"

Line 7: Line 7:
 
| width=50% valign='top' style='border: 1px solid black'|
 
| width=50% valign='top' style='border: 1px solid black'|
 
<partinfo>BBa_K731722 short</partinfo>
 
<partinfo>BBa_K731722 short</partinfo>
* This is the [[Part:B0010]] part extracted by PCR from the doulbe terminator [[Part:B0015]].
+
* This is the [[Part:BBa_B0010|B0010]] part extracted by PCR from the doulbe terminator [[Part:BBa_B0015|B0015]].
 
We didn't manage to extract it directly from the registry and we discovered that others had the same problem; for this reason we are submitting it once again, confirmed by sequencing and by experience...ready to use!
 
We didn't manage to extract it directly from the registry and we discovered that others had the same problem; for this reason we are submitting it once again, confirmed by sequencing and by experience...ready to use!
 
The characterization has been done both with T7 and Coli RNApolymerases.
 
The characterization has been done both with T7 and Coli RNApolymerases.

Revision as of 09:20, 24 August 2012

Terminator.png

biologyrrnBT1
directionForward
forward_efficiency0.976 +/- 0.005 with T7 RNAP (T7 promoter) 0.985 +/- 0.001 with E. coli RNAP (Ptac)

T1 terminator from E. coli rrnB

  • This is the B0010 part extracted by PCR from the doulbe terminator B0015.

We didn't manage to extract it directly from the registry and we discovered that others had the same problem; for this reason we are submitting it once again, confirmed by sequencing and by experience...ready to use! The characterization has been done both with T7 and Coli RNApolymerases.

Secondary Structure

File:Mfold-K731722-1.png


Measurement

  • [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]