Difference between revisions of "Part:BBa K731722:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | The terminator is in the standard backbone pSB1C3. | |
| − | + | Note: it is useful to use TBE1X instead of TAE1X to make screening gels of such small fragments. | |
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===Source=== | ===Source=== | ||
Revision as of 20:41, 23 August 2012
T1 terminator from E. coli rrnB
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The terminator is in the standard backbone pSB1C3. Note: it is useful to use TBE1X instead of TAE1X to make screening gels of such small fragments.
Source
This part comes from B0012 contained in E0840, a double terminator in which B0010 is the second one. We used an extraction PCR.