Difference between revisions of "Part:BBa K731480"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K731480 short</partinfo>
 
<partinfo>BBa_K731480 short</partinfo>
  
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This composite part is the same as BBa_K731400 but with the add of a sfGFP (superfolder GFP) downstream. This reporter gene is very useful to check and measure the expression of an upstream gene, that in this case codes for a cysteine desulfhydrase; its role is converting cysteine into pyruvate, ammonia and hydrogen sulfide.
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A sfGFP tagged CysDes inducible by IPTG.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 14:33, 23 August 2012

IPTG Inducible sfGFP tagged Cysteine desulfhydrase (CysDes)

A sfGFP tagged CysDes inducible by IPTG.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2460
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1559
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2490