Difference between revisions of "Part:BBa K731400:Design"

(→‎Design Notes)
(→‎Source)
Line 9: Line 9:
  
 
===Source===
 
===Source===
−
 
+
K731300 and K731600
−
LacI+LacIq promoter comes from pET-21b plasmid.
+
−
CysDes gene comes from the genome of Treponema denticola.
+
  
 
===References===
 
===References===

Revision as of 14:31, 23 August 2012

IPTG inducible Cysteine desulfhydrase (CysDes)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1560
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part was designed so that LacI transcription/translation does not affect the transcription/translation of CysDes. In other words LacI+lacIq promoter are in the reverse direction. Tac promoter + Lac operator + CysDes are in the forward direction.

Source

K731300 and K731600

References