Difference between revisions of "Part:BBa K731400:Design"

 
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===Design Notes===
 
===Design Notes===
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LacI+LacIq promoter is an autonomous part (K731300) also designed by our team.
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The part was designed so that LacI transcription/translation does not affect the transcription/translation of CysDes. In other words LacI+lacIq promoter are in the reverse direction. Tac promoter + Lac operator + CysDes are in the forward direction.
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===Source===
 
===Source===

Revision as of 14:31, 23 August 2012

IPTG inducible Cysteine desulfhydrase (CysDes)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1560
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part was designed so that LacI transcription/translation does not affect the transcription/translation of CysDes. In other words LacI+lacIq promoter are in the reverse direction. Tac promoter + Lac operator + CysDes are in the forward direction.

Source

LacI+LacIq promoter comes from pET-21b plasmid. CysDes gene comes from the genome of Treponema denticola.

References