Difference between revisions of "Part:BBa K731400"

 
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<partinfo>BBa_K731400 short</partinfo>
 
<partinfo>BBa_K731400 short</partinfo>
  
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This composite part was designed to construct a working CysDes gene under the control of an inducible promoter: the presence of IPTG inhibits the LacI repressor protein binding to the LacO operator; in this way it allows the RNApol to bind to the Ptac promoter and trascribe the gene of interest.
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This part is composed of K731300 and K731600. It is an IPTG inducible device regulating the expression of a Cysteine Desulfhydrase from Treponema Denticola (CysDes). This enzyme is an aminotransferase that converts cysteine into pyruvate, ammonia and hydrogen sulfide. The part has been successfully operated and characterized in psB1C3 and psB4K5.
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In this case the gene of interest is a Cysteine Desulfhydrase, an aminotransferase that converts cysteine into pyruvate, ammonia and hydrogen sulfide.
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<!-- Add more about the biology of this part here
 
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Revision as of 14:30, 23 August 2012

IPTG inducible Cysteine desulfhydrase (CysDes)

This part is composed of K731300 and K731600. It is an IPTG inducible device regulating the expression of a Cysteine Desulfhydrase from Treponema Denticola (CysDes). This enzyme is an aminotransferase that converts cysteine into pyruvate, ammonia and hydrogen sulfide. The part has been successfully operated and characterized in psB1C3 and psB4K5.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1560
  • 1000
    COMPATIBLE WITH RFC[1000]