Difference between revisions of "Part:BBa K731040"
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<partinfo>BBa_K731040 short</partinfo>
 
<partinfo>BBa_K731040 short</partinfo>
  
−
araC-pBAD regulating CysE M256 ([https://parts.igem.org/Part:BBa_K731030 K731030]) was fused at his 3' with sfGFP (spaced by a GSGSGS linker) using Gibson's Assembly.
+
A sfGFP tagged M256I CysE inducible by arabinose. This part was subcloned and characterized in pSB3C5.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 08:12, 23 August 2012

Inducible araC-pBAD promoter regulating a sfGFP-tagged M256I CysE

A sfGFP tagged M256I CysE inducible by arabinose. This part was subcloned and characterized in pSB3C5.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 2045
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1909
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 2075