Difference between revisions of "Part:BBa K731020:Design"

(→‎Source)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
−
CysE WT ([https://parts.igem.org/Part:BBa_K731010 K731010]) was inserted after araC-pBAD ([https://parts.igem.org/Part:BBa_K731200 K731200]) in his vector, cutting the former with S&P and the latter with X&P.
+
CysE WT ([https://parts.igem.org/Part:BBa_K731000 K731000]) was inserted after araC-pBAD ([https://parts.igem.org/Part:BBa_K731200 K731200]) in his vector, cutting the former with S&P and the latter with X&P.
  
 
===Source===
 
===Source===

Revision as of 08:34, 21 August 2012

Inducible araC-pBAD promoter regulating WT CysE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1909
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

CysE WT (K731000) was inserted after araC-pBAD (K731200) in his vector, cutting the former with S&P and the latter with X&P.

Source

K731200, K731000

References