Difference between revisions of "Part:BBa J119022"

Revision as of 14:44, 20 August 2012

HPP Bsa J23100 promoter in pSB1A2

J119022 can be used with either Part:BBa_J119044 or Part:BBa_J100091 for Golden Gate Assembly (GGA) to move promoter Part:BBa_J23100 to replace the transcriptional terminator (TT). J119022 is an ideal way to start learning how easy it is to swap two parts using [http://gcat.davidson.edu/mediawiki-1.15.0/index.php/Golden_Gate GGA.] You can follow a [http://gcat.davidson.edu/mediawiki-1.15.0/index.php/Golden_Gate_Assembly_protocol complete protocol for use in teaching labs.]

Part:BBa_J23100 promoter in pSB1A8 (Part:BBa_J119043) for BsaI/Ligase insertion into (Part:BBa_J119044), a modified version of Part:BBa_J100028.

This is the Part:BBa_J23100 promoter fragment designed for BsaI digestion and ligation into Part:BBa_J119044 as a positive control for use in Golden Gate Assembly. J119022 is the first promoter to be use as part of the [http://gcat.davidson.edu/RFP/ Registry of Functional Promoters].

J119022 is in pSB1A8 Part:BBa_J119043 with a BsaI site on the left reading right, with word CGAC being freed up, followed by Part:BBa_J23100 promoter, followed up by word GCGG which is freed up by the following BsaI site which reads to the left. The fragment is 57nt long after digestion. It should then be ligated into (Part:BBa_J119044) or a vector designed to accept the sticky end "Words" noted as "4NT Word" on the fragment diagram. (Requires a complement sticky end to be compatible).

Sequence and Features