Difference between revisions of "Part:BBa K734000"

Revision as of 16:45, 8 August 2012

Caffeine demethylation pathway

Synthetic operon consisting of methylxanthine N-demethylases: NdmA,NdmB, NdmC, and associated reductase NdmD from Psuedomonas putida CBB5. Also includes gst9 from Janthinobacterium sp. Marseille which is required for NdmC functionality. Constitutive expression is driven by promoter J23100.

Enables Escherichia coli cells to degrade caffeine (1,3,7-Methylxanthine) and related methylxanthines to xanthine.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 1229
Illegal XbaI site found at 1126
Illegal XbaI site found at 2229
Illegal XbaI site found at 3109
Illegal PstI site found at 548
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1229
Illegal NheI site found at 8
Illegal NheI site found at 31
Illegal NheI site found at 1730
Illegal NheI site found at 1925
Illegal PstI site found at 548
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1229
Illegal BglII site found at 2687
Illegal BglII site found at 3181
Illegal BglII site found at 3652
Illegal XhoI site found at 4793
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 1229
Illegal XbaI site found at 1126
Illegal XbaI site found at 2229
Illegal XbaI site found at 3109
Illegal PstI site found at 548
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1229
Illegal XbaI site found at 1126
Illegal XbaI site found at 2229
Illegal XbaI site found at 3109
Illegal PstI site found at 548
Illegal NgoMIV site found at 5491
Illegal NgoMIV site found at 5604
Illegal AgeI site found at 2224
Illegal AgeI site found at 3320
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 984
Illegal BsaI.rc site found at 2221

Revision as of 16:45, 8 August 2012 (view source) Erik.quandt (Talk \| contribs) ← Older edit		Revision as of 16:45, 8 August 2012 (view source) Erik.quandt (Talk \| contribs) Newer edit →
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	Synthetic operon consisting of methylxanthine N-demethylases: ''NdmA'',''NdmB'', ''NdmC'', and associated reductase ''NdmD'' from ''Psuedomonas putida CBB5''. Also includes ''gst9'' from ''Janthinobacterium sp. Marseille'' which is required for ''NdmC'' functionality. Constitutive expression is driven by promoter J23100.		Synthetic operon consisting of methylxanthine N-demethylases: ''NdmA'',''NdmB'', ''NdmC'', and associated reductase ''NdmD'' from ''Psuedomonas putida CBB5''. Also includes ''gst9'' from ''Janthinobacterium sp. Marseille'' which is required for ''NdmC'' functionality. Constitutive expression is driven by promoter J23100.

−	Enables ''Escherichia coli'' cells to degrade caffeine (1,3,7 Methylxanthine) and related methylxanthines to xanthine.	+	Enables ''Escherichia coli'' cells to degrade caffeine (1,3,7-Methylxanthine) and related methylxanthines to xanthine.