Difference between revisions of "Part:BBa J100085:Experience"
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This sequence is missing one nucleotide (T) from the GFP target spacer (part E0040). This nucleotide is number 683 in the E0040 sequence, and the T should be between 181 and 182 in part J100085 sequence. This caused the system to not destroy the plasmid containing GFP as it should. | This sequence is missing one nucleotide (T) from the GFP target spacer (part E0040). This nucleotide is number 683 in the E0040 sequence, and the T should be between 181 and 182 in part J100085 sequence. This caused the system to not destroy the plasmid containing GFP as it should. | ||
[[Image:CRISPR short.jpg|400px]] | [[Image:CRISPR short.jpg|400px]] | ||
| + | *CRISPR and GFP should have no green fluorescence | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 15:06, 23 July 2012
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_J100085
This sequence is missing one nucleotide (T) from the GFP target spacer (part E0040). This nucleotide is number 683 in the E0040 sequence, and the T should be between 181 and 182 in part J100085 sequence. This caused the system to not destroy the plasmid containing GFP as it should.
- CRISPR and GFP should have no green fluorescence
User Reviews
UNIQ75fdd1493eea5339-partinfo-00000000-QINU UNIQ75fdd1493eea5339-partinfo-00000001-QINU