Difference between revisions of "Part:BBa J100080"

Latest revision as of 15:31, 19 July 2012

Riboswitch and GFP

This part combines Riboswitch E (Part J100066) and superfolder GFP optimized for E. coli (Part J100070). It was assembled using the Golden Gate assembly Method and the BsaI sites that were engineered into the riboswitch and the superfolder GFP.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 100
Illegal AgeI site found at 187
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 175

@@ Line 2: / Line 2: @@
 <partinfo>BBa_J100080 short</partinfo>
-This part combines Riboswitch E ([https://parts.igem.org/Part:BBa_J100066 Part J100066]) and superfolder GFP optimized for E. coli ([https://parts.igem.org/Part:BBa_J10070 Part J100070). It was assembled using the Golden Gate assembly Method and the BsaI sites that were engineered into the riboswitch and the superfolder GFP.
+This part combines Riboswitch E ([https://parts.igem.org/Part:BBa_J100066 Part J100066]) and superfolder GFP optimized for E. coli ([https://parts.igem.org/Part:BBa_J10070 Part J100070]). It was assembled using the Golden Gate assembly Method and the BsaI sites that were engineered into the riboswitch and the superfolder GFP.
 <!-- Add more about the biology of this part here