Difference between revisions of "Part:BBa J100069:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_J100069 short</partinfo>
 
<partinfo>BBa_J100069 short</partinfo>
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===Design Notes===
 
===Design Notes===
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I used specifically designed PCR primers to add the Biobrick ends and the BsaI site.
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Designed to encode for superfolder GFP, specifically codon optimized for "E. coli".
  
  
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===Source===
 
===Source===
  
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Synthetic part using part I746916.
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===References===
 
===References===

Latest revision as of 17:55, 2 July 2012

Superfolder GFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 750
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 751
    Illegal PstI site found at 765
    Illegal NotI site found at 7
    Illegal NotI site found at 758
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 751
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 751
    Illegal PstI site found at 765
    Illegal AgeI site found at 54
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 23
    Illegal SapI.rc site found at 42


Design Notes

Designed to encode for superfolder GFP, specifically codon optimized for "E. coli".


Source

References