Difference between revisions of "Part:BBa K862003"
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This part was characterized by iGEM team Heidelberg_LSL 2012 in context of part <html> | This part was characterized by iGEM team Heidelberg_LSL 2012 in context of part <html> | ||
<a href="https://parts.igem.org/Part:BBa_K862002">BBa_K862002</a>. Please find the characterization details on the referred parts page. | <a href="https://parts.igem.org/Part:BBa_K862002">BBa_K862002</a>. Please find the characterization details on the referred parts page. | ||
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Latest revision as of 12:21, 25 June 2012
precB
The recB promoter sequence was taken for the E. coli MG1655 genome sequence (http://ecoliwiki.net/colipedia/index.php/recB:Gene). We assumed the main promoter region to be located from -70 to -1 bp upstream the recB start codon. Therefore this sequence was synthesized and cloned on an oligo basis.
We will not submit the physical DNA of this part, but we provide the oligo-sequences you can use for synthesizing and cloning this part in front of any EcoRI/XbaI precut reporter part.
RecB_fw: aattcgcggccgcttctagagCCTGAAGGCTGGAAAGTGTGGGAGAACGTCAGCGCGTTGCAGCAAACAATGCCCCTGATGAGTGAAAAGAc
RecB_rev: ctaggTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGctctagaagcggccgcg
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Characterization
This part was characterized by iGEM team Heidelberg_LSL 2012 in context of part BBa_K862002. Please find the characterization details on the referred parts page.