Difference between revisions of "Part:BBa K862000"
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===Characterisation=== | ===Characterisation=== | ||
| + | This part was characterized during the 2012 HS competition by the Heidelberg LSL team | ||
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<center><img src="https://static.igem.org/mediawiki/2012hs/8/80/Bildschirmfoto_2012-06-11_um_23.47.05.png" width="500"/></center> | <center><img src="https://static.igem.org/mediawiki/2012hs/8/80/Bildschirmfoto_2012-06-11_um_23.47.05.png" width="500"/></center> | ||
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<b>Fig. 1: ONPG assay of Bl21(DE3) irradiated for different times. </b>Both constructs show a strong correlation between the UV-irradiation time and the LacZ activity (production of o-nitrophenol). <br/> | <b>Fig. 1: ONPG assay of Bl21(DE3) irradiated for different times. </b>Both constructs show a strong correlation between the UV-irradiation time and the LacZ activity (production of o-nitrophenol). <br/> | ||
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| − | </p></html> | + | </p> |
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| + | <center><img src="https://static.igem.org/mediawiki/2012hs/c/c7/XGAL_Measurement.PNG" width="635"/></center> | ||
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| + | <b>Fig. 2: X-gal assay of Bl21(DE3) transformed with precA/precB/psulA_LacZ parts and irradiated for different times. </b>All constructs show a strong positive correlation between UV induction time and coloring of the wells. PrecA-LacZ gives the lowest reporter background expression whereas psulA-LacZ gives the highest overall coloring of the samples.</b> | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 15:58, 24 June 2012
precA-LacZ-doubleTerminator
This is a part for the precise quantification of UV-radiation or radioactive radiation in E. coli (recA+) strains, i.e. BL21(DE3). It consits of a recA Promoter (part BBa_J22106) fused to a LacZ reporter cloned in front of a double terminator (part BBa_K173004).
Characterisation
This part was characterized during the 2012 HS competition by the Heidelberg LSL team
Fig. 1: ONPG assay of Bl21(DE3) irradiated for different times. Both constructs show a strong correlation between the UV-irradiation time and the LacZ activity (production of o-nitrophenol).
Fig. 2: X-gal assay of Bl21(DE3) transformed with precA/precB/psulA_LacZ parts and irradiated for different times. All constructs show a strong positive correlation between UV induction time and coloring of the wells. PrecA-LacZ gives the lowest reporter background expression whereas psulA-LacZ gives the highest overall coloring of the samples.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]