Difference between revisions of "Part:BBa K727777"

 
 
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<partinfo>BBa_K727777 short</partinfo>
 
<partinfo>BBa_K727777 short</partinfo>
  
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FMO3 (flavin monooxygenase 3) is an enzyme that catalyses the oxidation of TMA (trimethylamine) into TMAO (trimethylamine oxide) which results as the removal of unpleasant fish odor.
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FMO3 (flavin monooxygenase 3) is an enzyme that catalyses the oxidation of TMA (trimethylamine) into TMAO (trimethylamine oxide) which results as the removal of unpleasant fish odor.We added Lux cassette right promoter that up-regulated by HSL and LuxR in the course of being able to regulate easily. We intended to generate a positive feedback response with the help of producing LuxR and HSL and activating the promoter with them.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 22:54, 16 June 2012

FMO3 Generator-Complete system

FMO3 (flavin monooxygenase 3) is an enzyme that catalyses the oxidation of TMA (trimethylamine) into TMAO (trimethylamine oxide) which results as the removal of unpleasant fish odor.We added Lux cassette right promoter that up-regulated by HSL and LuxR in the course of being able to regulate easily. We intended to generate a positive feedback response with the help of producing LuxR and HSL and activating the promoter with them.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 64
    Illegal suffix found in sequence at 1680
    Illegal PstI site found at 1233
    Illegal PstI site found at 1596
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 64
    Illegal SpeI site found at 1681
    Illegal PstI site found at 1233
    Illegal PstI site found at 1596
    Illegal PstI site found at 1695
    Illegal NotI site found at 70
    Illegal NotI site found at 1688
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 64
    Illegal BglII site found at 3130
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 64
    Illegal suffix found in sequence at 1681
    Illegal PstI site found at 1233
    Illegal PstI site found at 1596
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 64
    Illegal XbaI site found at 79
    Illegal SpeI site found at 1681
    Illegal PstI site found at 1233
    Illegal PstI site found at 1596
    Illegal PstI site found at 1695
    Illegal NgoMIV site found at 947
    Illegal AgeI site found at 447
    Illegal AgeI site found at 926
    Illegal AgeI site found at 1068
  • 1000
    COMPATIBLE WITH RFC[1000]