Difference between revisions of "Part:BBa J100066:Design"
 
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===Design Notes===
 
===Design Notes===
−
None
+
Designed to permit translation in the presence of theophylline.
  
  

Latest revision as of 13:52, 12 June 2012

Synthetic Riboswitch


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 196
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 197
    Illegal PstI site found at 211
    Illegal NotI site found at 7
    Illegal NotI site found at 204
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 197
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 197
    Illegal PstI site found at 211
    Illegal AgeI site found at 122
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 190


Design Notes

Designed to permit translation in the presence of theophylline.


Source

Synthetic part taken from reference below.

References

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2988590/?tool=pubmed