Difference between revisions of "Part:BBa K896936:Design"
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===Source=== | ===Source=== | ||
| − | + | PCR'd from Escherichia coli str. K-12 substr. MG1655 wildtype phage. | |
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===References=== | ===References=== | ||
Latest revision as of 18:28, 27 April 2012
Human haptoglobin gene recombinent on Ecoli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 482
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 972
Design Notes
Primers used in the PCR:
Hp_E_FP: gcttctag atgagtgccctgggag (24nt,16nt,TM:54C,%GC:63%) Hp_E_RP: gct ctgcagcggccgctactagta ttagttctcagctatggtcttctg (48nt,24nt,TM:55C,%GC:42%)
Digested the PCR product with XbaI and PstI, and ligated the digestion product with a gel purified XbaI,PstI digested pSB1A2 plasmid backbone.
Source
PCR'd from Escherichia coli str. K-12 substr. MG1655 wildtype phage.