Difference between revisions of "Part:BBa J176005"

Revision as of 21:14, 5 April 2012

mCh

Codon optimized mCherry red fluorescent protein

Usage and Biology

Protein Data Bank ID 2H5Q

"Monomeric cherry" (mCherry) red fluorescent protein is a derivative of monomeric RFP from Discosoma sp. (Shaner et al. 2004, Shu et al. 2006). This BioBrick was codon optimized (by the lab of Pam Silver) for expression in human/ mammalian cells. Only a single copy is needed for detectable signal. We have assembled two copies in tandem to create a "bulky" protein for use as an artificial transcriptional repressor (Haynes et al. 2012).

REFERENCES

Shaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, Tsien RY (2004) Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat Biotechnol. 22:1567-1572
Shu X, Shaner NC, Yarbrough CA, Tsien RY, Remington SJ (2006) Novel chromophores and buried charges control color in mFruits. Biochem. 45:9639-9647.
Haynes KA, Ceroni F, Flicker D, Younger A, Silver PA. (2012) A sensitive switch for visualizing natural gene silencing in single cells. ACS Syn Biol.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 9: / Line 9: @@
 * Protein Data Bank ID 2H5Q
-This "monomeric cherry" (mCherry) red fluorescent protein (described in Shu et al., 2006) was codon optimized (by the lab of Pam Silver) for expression in human/ mammalian cells. Only a single copy is needed for detectable signal. We have assembled two copies in tandem to create a "bulky" protein for use as an artificial transcriptional repressor (Haynes et al. 2012).
+"Monomeric cherry" (mCherry) red fluorescent protein is a derivative of monomeric RFP from Discosoma sp. (Shaner et al. 2004, Shu et al. 2006). This BioBrick was codon optimized (by the lab of Pam Silver) for expression in human/ mammalian cells. Only a single copy is needed for detectable signal. We have assembled two copies in tandem to create a "bulky" protein for use as an artificial transcriptional repressor (Haynes et al. 2012).
 REFERENCES
+# Shaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, Tsien RY (2004) Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat Biotechnol. 22:1567-1572
 # Shu X, Shaner NC, Yarbrough CA, Tsien RY, Remington SJ (2006) Novel chromophores and buried charges control color in mFruits. Biochem. 45:9639-9647.
 # Haynes KA, Ceroni F, Flicker D, Younger A, Silver PA. (2012) A sensitive switch for visualizing natural gene silencing in single cells. ACS Syn Biol.