Difference between revisions of "Part:BBa J176005"

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* Protein Data Bank ID 2H5Q
 
* Protein Data Bank ID 2H5Q
  
This "monomeric cherry" (mCherry) red fluorescent protein is codon optimized for expression in human/ mammalian cells. Only a single copy is needed for detectable signal. We have assembled two copies in tandem to create a "bulky" protein for use as an artificial transcriptional repressor (Haynes et al. 2012).
+
This "monomeric cherry" (mCherry) red fluorescent protein (described in Shu et al., 2006) was codon optimized (by the lab of Pam Silver) for expression in human/ mammalian cells. Only a single copy is needed for detectable signal. We have assembled two copies in tandem to create a "bulky" protein for use as an artificial transcriptional repressor (Haynes et al. 2012).
  
  
 
REFERENCES
 
REFERENCES
* Haynes KA, Ceroni F, Flicker D, Younger A, Silver PA. (2012) A sensitive switch for visualizing natural gene silencing in single cells. ACS Syn Biol.
+
# Shu X, Shaner NC, Yarbrough CA, Tsien RY, Remington SJ (2006) Novel chromophores and buried charges control color in mFruits. Biochem. 45:9639-9647.
 +
# Haynes KA, Ceroni F, Flicker D, Younger A, Silver PA. (2012) A sensitive switch for visualizing natural gene silencing in single cells. ACS Syn Biol.
  
  

Revision as of 21:07, 5 April 2012

mCh

Codon optimized mCherry red fluorescent protein


Usage and Biology

  • Protein Data Bank ID 2H5Q

This "monomeric cherry" (mCherry) red fluorescent protein (described in Shu et al., 2006) was codon optimized (by the lab of Pam Silver) for expression in human/ mammalian cells. Only a single copy is needed for detectable signal. We have assembled two copies in tandem to create a "bulky" protein for use as an artificial transcriptional repressor (Haynes et al. 2012).


REFERENCES

  1. Shu X, Shaner NC, Yarbrough CA, Tsien RY, Remington SJ (2006) Novel chromophores and buried charges control color in mFruits. Biochem. 45:9639-9647.
  2. Haynes KA, Ceroni F, Flicker D, Younger A, Silver PA. (2012) A sensitive switch for visualizing natural gene silencing in single cells. ACS Syn Biol.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]