Difference between revisions of "Part:BBa J176005"

Revision as of 21:07, 5 April 2012

mCh

Codon optimized mCherry red fluorescent protein

Usage and Biology

Protein Data Bank ID 2H5Q

This "monomeric cherry" (mCherry) red fluorescent protein (described in Shu et al., 2006) was codon optimized (by the lab of Pam Silver) for expression in human/ mammalian cells. Only a single copy is needed for detectable signal. We have assembled two copies in tandem to create a "bulky" protein for use as an artificial transcriptional repressor (Haynes et al. 2012).

REFERENCES

Shu X, Shaner NC, Yarbrough CA, Tsien RY, Remington SJ (2006) Novel chromophores and buried charges control color in mFruits. Biochem. 45:9639-9647.
Haynes KA, Ceroni F, Flicker D, Younger A, Silver PA. (2012) A sensitive switch for visualizing natural gene silencing in single cells. ACS Syn Biol.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 9: / Line 9: @@
 * Protein Data Bank ID 2H5Q
-This "monomeric cherry" (mCherry) red fluorescent protein is codon optimized for expression in human/ mammalian cells. Only a single copy is needed for detectable signal. We have assembled two copies in tandem to create a "bulky" protein for use as an artificial transcriptional repressor (Haynes et al. 2012).
+This "monomeric cherry" (mCherry) red fluorescent protein (described in Shu et al., 2006) was codon optimized (by the lab of Pam Silver) for expression in human/ mammalian cells. Only a single copy is needed for detectable signal. We have assembled two copies in tandem to create a "bulky" protein for use as an artificial transcriptional repressor (Haynes et al. 2012).
 REFERENCES
-* Haynes KA, Ceroni F, Flicker D, Younger A, Silver PA. (2012) A sensitive switch for visualizing natural gene silencing in single cells. ACS Syn Biol.
+# Shu X, Shaner NC, Yarbrough CA, Tsien RY, Remington SJ (2006) Novel chromophores and buried charges control color in mFruits. Biochem. 45:9639-9647.
+# Haynes KA, Ceroni F, Flicker D, Younger A, Silver PA. (2012) A sensitive switch for visualizing natural gene silencing in single cells. ACS Syn Biol.