Difference between revisions of "Part:BBa J176005"
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===Usage and Biology===
 
===Usage and Biology===
  
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This "monomeric cherry" (mCherry) red fluorescent protein is codon optimized for expression in human/ mammalian cells. Only a single copy is needed for detectable signal. We have assembled two copies in tandem to create a "bulky" protein for use as an artificial transcriptional repressor.
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* Protein Data Bank ID 2H5Q
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This "monomeric cherry" (mCherry) red fluorescent protein is codon optimized for expression in human/ mammalian cells. Only a single copy is needed for detectable signal. We have assembled two copies in tandem to create a "bulky" protein for use as an artificial transcriptional repressor (Haynes et al. 2012).
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REFERENCES
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* Haynes KA, Ceroni F, Flicker D, Younger A, Silver PA. (2012) A sensitive switch for visualizing natural gene silencing in single cells. ACS Syn Biol.
  
  

Revision as of 20:59, 5 April 2012

mCh

Codon optimized mCherry red fluorescent protein


Usage and Biology

  • Protein Data Bank ID 2H5Q

This "monomeric cherry" (mCherry) red fluorescent protein is codon optimized for expression in human/ mammalian cells. Only a single copy is needed for detectable signal. We have assembled two copies in tandem to create a "bulky" protein for use as an artificial transcriptional repressor (Haynes et al. 2012).


REFERENCES

  • Haynes KA, Ceroni F, Flicker D, Younger A, Silver PA. (2012) A sensitive switch for visualizing natural gene silencing in single cells. ACS Syn Biol.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]