Difference between revisions of "Part:BBa J176003"

 
 
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Ubc constitutive promoter
 
Ubc constitutive promoter
  
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===Usage and Biology===
 
===Usage and Biology===
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[[Image:Haynes_Promoters_mCh.png|thumb|400px|'''Comparison of human promoters in U2OS cells''']]
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* "Weak" expression compared to CMV
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* Constitutive (does not require induction)
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The Ubiquitin C (Ubc) promoter drives expression at a level below that of the human phosphoglycerate kinase (HPK) promoter in mammalian cells. Expression of red fluorescent protein and cyan fluorescent protein are difficult to detect via microscopy, but can be measured at levels above background (non-fluorescent cells) via flow cytometry with some adjustment of instrument sensitivity.
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<br><br>
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REFERENCES
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* Will be added soon
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Latest revision as of 20:28, 5 April 2012

Ubc

Ubc constitutive promoter


Usage and Biology

Comparison of human promoters in U2OS cells
  • "Weak" expression compared to CMV
  • Constitutive (does not require induction)

The Ubiquitin C (Ubc) promoter drives expression at a level below that of the human phosphoglycerate kinase (HPK) promoter in mammalian cells. Expression of red fluorescent protein and cyan fluorescent protein are difficult to detect via microscopy, but can be measured at levels above background (non-fluorescent cells) via flow cytometry with some adjustment of instrument sensitivity.

REFERENCES

  • Will be added soon


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]