Difference between revisions of "Part:BBa J176028"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | [[Image:Haynes_Promoters_mCh.png|thumb | + | [[Image:Haynes_Promoters_mCh.png|thumb|400px|'''Comparison of human promoters in U2OS cells''']] |
* "Medium" strength expression compared to CMV | * "Medium" strength expression compared to CMV | ||
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The human phosphoglycerate kinase (HPK) promoter drives expression at a level intermediate to the ubiquitin C promoter (Ubc) and the popular cytomegalovirus promoter (CMV) in mammalian cells. | The human phosphoglycerate kinase (HPK) promoter drives expression at a level intermediate to the ubiquitin C promoter (Ubc) and the popular cytomegalovirus promoter (CMV) in mammalian cells. | ||
| + | <br><br> | ||
REFERENCES | REFERENCES | ||
Revision as of 20:21, 5 April 2012
HPK
Constitutive human phosphoglycerate kinase promoter
Usage and Biology
- "Medium" strength expression compared to CMV
- Constitutive (does not require induction)
The human phosphoglycerate kinase (HPK) promoter drives expression at a level intermediate to the ubiquitin C promoter (Ubc) and the popular cytomegalovirus promoter (CMV) in mammalian cells.
REFERENCES
- Will be added soon
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 116
- 1000COMPATIBLE WITH RFC[1000]