Difference between revisions of "Part:BBa J176013"
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[[Image:KAH_VP64.png|thumb|left|350px|'''The VP64 Activation Domain Module''' is often fused to DNA binding domains to make strong synthetic transcription factors. Figure adapted from Beerli et al., 1998.]] | [[Image:KAH_VP64.png|thumb|left|350px|'''The VP64 Activation Domain Module''' is often fused to DNA binding domains to make strong synthetic transcription factors. Figure adapted from Beerli et al., 1998.]] | ||
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* Mammalian expression vector required | * Mammalian expression vector required | ||
* Protein domain; requires promoter, start codon, stop codon, and polyA signal for proper expression | * Protein domain; requires promoter, start codon, stop codon, and polyA signal for proper expression | ||
Revision as of 20:16, 5 April 2012
VP64
Tetrameric VP16 transcription activator domain
Usage and Biology
- Mammalian expression vector required
- Protein domain; requires promoter, start codon, stop codon, and polyA signal for proper expression
- VP64 doesn't do much as a free-floating domain. Fuse it to a DNA binding domain for the best results
VP64 is a transcriptional activator composed of four tandem copies of VP16 (Herpes Simplex Viral Protein 16, amino acids 437-447, DALDDFDLDML) connected with glycine-serine linkers. When fused to another protein domain that can bind near the promoter of a gene, VP64 acts as a strong transcriptional activator. This module is a classic molecular biology tool, which pre-dates fancy terms like "synthetic biology." See the References section for some history on VP16 and VP64.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]